COLLOIDAL SILVER has been shunned as a possible TREATMENT for serious disease conditions, even friends have scoffed at it as ludicrous and “how dare I express an opinion” as I am not a doctor! However I still have some basic COMMON SENSE which is not so common any more. As we look at the way the public have been so willing to trash their FREEDOMS and worship our dictator who is treashing our economy and our future…it is just so UNBELIEVABLE.
We all know it is not in the interest of vested interests who rely on the development and manufacture of DRUGS and VACCINES to make the big $$$$$$$. SILVER NANO PARTICLES are too simple and too inexpensive to gain anyone’s interest. However in recent months there has been renewed interest and we see some great research establishing this form of treatment as a viable option.
Indeed we have seen its effectiveness for years as many folk have used it to combat the normal Influenza and other disease conditions. It is more than effective according to many users and it is hoped by making these research papers more widely known to the public, that this and other TREATMENTS can become widely utilized so that the insane and inadequate directive to simply GO HOME AND ISOLATE is changed to GO HOME AND GET TREATED by what ever means proves to be effective.
A new protocol needs to be developed so that every POSITIVE CASE IS IMMEDIATELY TREATED by at least good and well established IMMUNE BOOSTERS AND ACCEPTED TREATMENTS. Way too much damage has been done to all our lives and we need to urgently get back to NORMAL LIFE as soon as possible and and stop wasting the future of our younger folk and steeling precious time from our older folk. LIFE IS TOO PRECIOUS!
REFERENCES – SILVER NANO PARTICLES
Antiviral activity of silver nanoparticle/chitosan composites against H1N1 influenza A virus
Yasutaka Mori, Takeshi Ono, Yasushi Miyahira, Vinh Quang Nguyen, Takemi Matsui & Masayuki Ishihara
Nanoscale Research Letters volume 8, Article number: 93 (2013)
10.1186/1556-276X-8-93 REF: https://nanoscalereslett.springeropen.com/articles/10.1186/1556-276X-8-93 Citation link to read full article
Antiviral and immunomodulatory activity of silver nanoparticles in experimental rsv infection
Dorothea Morris, Maria Ansar, Janice Speshock, Teodora Ivanciuc, Yue Qu, Antonella Casola, Roberto Garofalo
10.3390/v11080732
Pediatrics
Chapter 15: Silver Nanoparticles as Novel Antibacterial and Antiviral Agents
Stefania Galdiero, Annarita Falanga, Marco Cantisani, Avinash Ingle, Massimiliano Galdiero and Mahendra Rai
https://doi.org/10.1142/9789814520652_0015 Cited by:2
Advanced and innovative technologies are continuously dedicated to the understanding of the mechanisms of diseases and to the design of new drugs, but infectious diseases continue to be one of the greatest health burdens worldwide. The main drawbacks for conventional antimicrobial agents are the development of multiple drug resistance and adverse side effects. Advances in nanotechnology have produced novel horizons in nanomedicine, enabling the synthesis of nanoparticles that could be assembled into complex architectures. Among these, silver nanoparticles are attracting great interest owing to their potent antibacterial and antiviral activity. This chapter summarizes emerging efforts to tackle the current challenges in treating infectious diseases, particularly using antimicrobial silver nanoparticles. Silver nanoparticles toxicity is also addressed to disclose the concerns against widespread application in the medical field. end abstract
COVID-19: Pathophysiology, treatment options, nanotechnology approaches, and research agenda to combating the SARS-CoV2 pandemichttps://www.researchgate.net/publication/343822846
ABSTRACT: The recent corona virus disease (COVID-19) outbreak has claimed the lives of many around the world and highlighted an urgent need for experimental strategies to prevent, treat and eradicate the virus. COVID-19, an infectious disease caused by a novel corona virus and no approved specific treatment is available yet. A vast number of promising antiviral treatments involving nanotechnology are currently under investigation to aid in the development of COVID-19 drug delivery. The prospective treatment options integrating the ever-expanding field of nanotechnology have been compiled, with the objective to show that these can be potentially developed for COVID-19 treatment. This review summarized the current state of knowledge, research priorities regarding the pandemic and post COVID-19. We also focus on the possible nanotechnology approaches that have proven to be successful against other viruses and the research agenda to combat COVID-19.
Critical Reviews in Microbiology. Volume 42, 2016 – Issue 1
Metal nanoparticles: The protective nanoshield against virus infection
Mahendra Rai,Shivaji D. Deshmukh,Avinash P. Ingle,Indarchand R. Gupta,Massimiliano Galdiero &Stefania Galdiero
Pages 46-56 | Received 24 Oct 2013, Accepted 30 Dec 2013, Published online: 22 Apr 2014
https://doi.org/10.3109/1040841X.2013.879849
Abstract Re-emergence of resistance in different pathogens including viruses are the major cause of human disease and death, which is posing a serious challenge to the medical, pharmaceutical and biotechnological sectors. Though many efforts have been made to develop drug and vaccines against re-emerging viruses, researchers are continuously engaged in the development of novel, cheap and broad-spectrum antiviral agents, not only to fight against viruses but also to act as a protective shield against pathogens attack. Current advancement in nanotechnology provides a novel platform for the development of potential and effective agents by modifying the materials at nanolevel with remarkable physicochemical properties, high surface area to volume ratio and increased reactivity. Among metal nanoparticles, silver nanoparticles have strong antibacterial, antifungal and antiviral potential to boost the host immunity against pathogen attack. Nevertheless, the interaction of silver nanoparticles with viruses is a largely unexplored field. The present review discusses antiviral activity of the metal nanoparticles, especially the mechanism of action of silver nanoparticles, against different viruses such HSV, HIV, HBV, MPV, RSV, etc. It is also focused on how silver nanoparticles can be used in therapeutics by considering their cytotoxic level, to avoid human and environmental risks. end abstract
ANTIBACTERIAL AND ANTIVIRAL PROPERTIES OF SILVER NANOPARTICLES SYNTHESIZED BY MARINE ACTINOMYCETES
Janardhan Avilala and Narasimha Golla *
Department of Virology, Applied Microbiology Laboratory, Sri Venkateswara University, Tirupati – 517503, Andhra Pradesh, India.
DOI: 10.13040/IJPSR.0975-8232.10(3).1223-28
https://ijpsr.com/bft-article/antibacterial-and-antiviral-properties-of-silver-nanoparticles-synthesized-by-marine-actinomycetes/?view=fulltext
ABSTRACT: Present work has been conducted to evaluate antibacterial and antiviral activity of silver nanoparticles using marine Actinomycete, Nocardiopsis alba, isolated from mangrove soil. Formation, size, and shapes of silver nanoparticles (AgNPs) were characterized by UV-visible spectroscopy, X-Ray Diffraction (XRD), FTIR and Transmission electron microscope (TEM). From the UV-visible spectroscopy, the absorption peak was found at 420 nm. The SEM images confirmed that the sample contains spherical silver nanoparticles. The XRD analysis confirmed that the silver nanoparticles are crystalline, which was confirmed by the FT-IR peak at 564 cm-1 corresponding to the silver nanoparticles vibration present in the crystalline structure. The silver nanoparticles have significant antibacterial activity against Pseudomonas aeruginosa, Klebsiella pneumonia, Streptococcus aureus, and E. coli. and antiviral activity against new castle viral disease (NDV) in cattle The findings of the present study suggest that the silver nanoparticles possess a good antibacterial a d antiviral activity and could have great importance as a therapeutic agent in current nanomedicine.
Antiviral properties of silver nanoparticles against norovirus surrogates and their efficacy in coated, polyhydroxyalkanoates systems, J.L. Castro-Mayorga1, W. Randazzo1, M.J. Fabra1, J.M. Lagaron1 5, R. Aznar1,2, G. Sánchez1,
ABSTRACT: Silver nanoparticles (AgNP) have strong broad-spectrum antimicrobial activity and gained increased attention for the development of AgNP based products, including medical andfood applications. Initially, the efficacy of AgNP and silver nitrate (AgNO3) was evaluated for inactivating norovirus surrogates, the feline calicivirus (FCV) and the murine norovirus (MNV). These norovirus surrogates were exposed to AgNO3 and AgNP solutions for 24 h at 25°C and then analyzed by cell-culture assays. Both AgNP and silver ions significantly decreased FCV and MNV infectivity in a dose-dependent manner between concentrations of 2.1 and 21 mg/L. Furthermore, poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) films were prepared by depositing a coating of thermally post-processed electrospun PHBV18 /AgNP fiber mats over compression moulded PHBV3 films. After 24 h exposure at 37°C and 100% RH, no infectious FCV were recovered when in contact with the AgNP films while MNV titers decreased by 0.86 log. The morphology of the PHBV18 and PHBV18/AgNP fibers studied by SEM showed smooth and continuous fibers in both cases and the EDAX analysis confirmed the homogeneously distribution of AgNP into the coating and onto the PHBV3/PHBV18 layer. This study showed, for the first time, the suitability of the PHBV18/AgNP electrospun coating for antiviral surfaces. END ABSTRACT
https://phys.org/news/2020-07-fabrics-antiviral-properties.html
EXTRACT: Brazilian textile companies have begun producing and marketing fabrics treated with silver and silica nanoparticles developed by the research groups at the Center for Functional Materials (CDMF) and the Theoretical and Computational Chemistry Laboratory (QTC) at the Universitat Jaume I, in collaboration with the company Nanox Tecnologia, which provide the new fabrics with antibacterial, antifungal and antiviral properties with 99.99% effectiveness.
Guilherme C. Tremiliosi et al. Ag nanoparticles-based antimicrobial polycotton fabrics to prevent the transmission and spread of SARS-CoV-2, (2020). DOI: 10.1101/2020.06.26.152520
https://phys.org/news/2020-06-fabric-capable-sars-cov-contact.html
Silver Nanoparticles with Bronchodilators Through Nebulisation to Treat COVID 19 Patients.Dr. Subhasish Sarkar
JOURNAL OF CURRENT MEDICAL RESEARCH AND OPINION an Open Access Publication ISSN: (O) 2589-8779 | (P) 2589-8760
Abstract: There are no effective antiviral drugs available against the pandemic causing COVID 19 virus. The complex protein – protein interaction between the virus and host is yet to be determined for designing of precise antiviral drugs against corona virus. The pandemic had claimed several thousand lives and had resulted most devastating effects on our mankind. In this scenario a potent drug is needed which will kill the viruses with minimal side effects on human body. Here I propose a novel antiviral therapy for effective killing of COVID19 with minimal side effects.
Applications of Silver nanoparticles in diverse sectors
Abstract:The review article summarizes the applications of silver nanoparticles for diverse sectors. Over the decades, nanoparticles used as dignified metals such as silver exhibited distinctive characteristics basically correlated to chemical, physical and biological property of counterparts having bulkiness. Numerous studies reported that Nanoparticles of about 100nm diameter play a crucial role in widely spread industries due to unique properties including the dimension of small particle, high surface area and quantum confinement and they dispersed without agglomeration. Decade of discoveries clearly established that shape, size and distribution of Silver nanoparticles strongly affect the electromagnetic, optical and catalytic properties, which are often an assortment of changeable synthetic methods and reducing agents with stabilizers. Generation after generation the postulates come forth about properties of silver for the ancient Greeks cook from silver pots and the old adage ‘born with a silver spoon in his mouth’ thus show that eating with a silver spoon was well-known as uncontaminated. Impregnation of metals with silver nanoparticles is a practical way to exploit the microbe aggressive properties of silver at very low cost. The nanoparticles help in targeted delivery of drugs, enhancing bioavailability, sustaining drug or gene effect in target tissues, and enhancing the stability. Implementations of silver partials in medical science and biological science have been noticed from years ago; however alteration with nanotechnology is innovative potential. Over 23% of all nanotechnology based products, diagnostic and therapeutic applications implanted with silver nanoparticles (e.g. In arthritic disease and wound healing, etc.) and widely known for their antifungal, antibacterial, antiviral effect, employed in textile fabrics and added into cosmetic products as antiseptic to overcome skin problems. Thus, Silver nanoparticles (AgNPs) have been urbanized as an advanced artifact in the field of nanotechnology.
This paper is very comprehensive in covering a very wide range of applications. Of particular interest is its ANTI VIRAL characteristics so I have included that section here: Antiviral accomplishment– Viral mediated diseases are common and becoming more prominent worldwide; therefore, developing anti-viral agents is essential. The mechanisms of the antiviral activity of AgNPs are an important aspect in antiviral therapy. AgNPs have unique interactions with bacteria and viruses based on certain size ranges and shapes [27, 41, 59]. Due to the antiviral activity, nano-Ag incorporated into polysulfone ultrafiltration membranes; (nAg-PSf) was evaluated against MS2 bacteriophage, which shows the significant antiviral activity, due to increased membrane hydrophilicity [60]. AgNPs have demonstrated efficient inhibitory activities against human immunodeficiency virus (HIV) and hepatitis B virus (HBV) [61]. A study focused towards the antiviral action of the AgNPs; the information confirm that each scavenger cell (M)-tropic and T-lymphocyte (T)-tropic strains of HIV-1 were sensitive to the AgNP-coated polyurethane condom (PUC) [62]. Even though, numerous studies have revealed that AgNPs could slow down the viability of viruses nevertheless the exact mechanism of antiviral activity is still obscure. The studies by Trefry and Wooley found that AgNPs caused a four- to five-log reduction in infective agent titre at concentrations that weren’t cytotoxic to cells [63]. Interestingly, in the presence of AgNPs, virus was capable of adsorbtion to cells, and this viral entry is responsible for the antiviral effects of AgNPs. The Hemagglutination assay indicated that AgNPs might considerably inhibit the growth of the influenza virus in Madin-Darby canine urinary organ cells i.e. kidney. The study as of intranasal AgNPs organization within mice considerably improved survival, lower lung viral titer levels, minor pathologic lesions in lung tissue, and remarkable survival advantage after infection with the H3N2 influenza virus, suggesting that AgNPs had a significant role in mice survival [64].
Biologically-synthesized AgNPs inhibited the viability of herpes simplex virus (HSV) types 1 and 2 and human para-influenza virus type 3, based on size and zeta potential of AgNPs [65]. The treatment of Vero cells with “non-cytotoxic” concentrations of AgNPs considerably repressed the replication of “Peste des petits ruminant’s virus” (PPRV). The mechanisms of viral replication are due to the interaction of AgNPs with the virion surface and the virion core [66]. Tannic acid synthesized the assorted sizes AgNPs that is able to reduce “HSV-2” infectivity in vitro and in vivo through direct interaction, blocked virus attachment, penetration, and further spread [67]. The antiviral property of Ag+ alone or combination with 50 ppb Ag+ and 20 ppm CO32- (carbonate ions) was performed on bacteriophage MS2 phage. The results from this study evaluate that 50 ppb Ag+ alone was unable to affect the phage, and the combination of 50 ppb Ag+ and 20 ppm CO3 was found to have an effective antiviral property within a contact time of 15 min [68].
APPLICATION OF SILVER NANOPARTICLES IN VIRAL INHIBITION: A NEW HOPE FOR ANTIVIRALS
- Conclusion: In this era, when drug and vaccine development for the removal of various viral diseases is
riding high, some viral strains have emerged that are resistant against the drugs and vaccines, like
HIV. So it is important to introduce the multidisciplinary approaches with the classical
epidemiology, along with the clinical phases to introduce a new drug or vaccine which proves
highly beneficial against the resistant strain. Nanotechnology is the one that gives the opportunity
to re-discover biological properties of ancient antimicrobial and antiviral compounds.
Nanoparticles, mainly silver have antiviral activities against the many viruses of today that are
playing havoc with lives worldwide. Extensive research and clinical trials need to be carried out so
as to accentuate the efficacy of this medical marvel towards betterment of the health of the global
population. N. KHANDELWAL* , G. KAUR, N. KUMARa, A. TIWARI
Mode of antiviral action of silver nanoparticles against HIV-1
Abstract – Background: Silver nanoparticles have proven to exert antiviral activity against HIV-1 at non-cytotoxic concentrations, but the mechanism underlying their HIV-inhibitory activity has not been not fully elucidated. In this study, silver nanoparticles are evaluated to elucidate their mode of antiviral action against HIV-1 using a panel of different in vitro assays.
Results: Our data suggest that silver nanoparticles exert anti-HIV activity at an early stage of viral replication, most likely as a virucidal agent or as an inhibitor of viral entry. Silver nanoparticles bind to gp120 in a manner that prevents CD4-dependent virion binding, fusion, and infectivity, acting as an effective virucidal agent against cell-free virus (laboratory strains, clinical isolates, T and M tropic strains, and resistant strains) and cell-associated virus. Besides, silver nanoparticles inhibit post-entry stages of the HIV-1 life cycle.
Conclusions: These properties make them a broad-spectrum agent not prone to inducing resistance that could be used preventively against a wide variety of circulating HIV-1 strains.
Mode of antiviral action of silver nanoparticles against HIV-1
Abstract: BACKGROUND: Silver nanoparticles have proven to exert antiviral activity against HIV-1 at non-cytotoxic concentrations, but the mechanism underlying their HIV-inhibitory activity has not been not fully elucidated. In this study, silver nanoparticles are evaluated to elucidate their mode of antiviral action against HIV-1 using a panel of different in vitro assays. RESULTS: Our data suggest that silver nanoparticles exert anti-HIV activity at an early stage of viral replication, most likely as a virucidal agent or as an inhibitor of viral entry. Silver nanoparticles bind to gp120 in a manner that prevents CD4-dependent virion binding, fusion, and infectivity, acting as an effective virucidal agent against cell-free virus (laboratory strains, clinical isolates, T and M tropic strains, and resistant strains) and cell-associated virus. Besides, silver nanoparticles inhibit post-entry stages of the HIV-1 life cycle. CONCLUSIONS: These properties make them a broad-spectrum agent not prone to inducing resistance that could be used preventively against a wide variety of circulating HIV-1 strains.
How-Nanoparticles-Bee-Venom-Destroy-HIV – Microscopic nanoparticles have unique and exciting properties. In biomedicine, they are used to transport important proteins throughout the body. Bee venom’s principle toxin is melittin, a small protein. Researchers used nanoparticles to distribute melittin in laboratory studies…….
Applications of nanoparticle systems in drug delivery technology–ABSTRACT: The development of nanoparticle-based drug formulations has yielded the opportunities to address and treat challenging diseases. Nanoparticles vary in size but are generally ranging from 100 to 500 nm. Through the manipulation of size, surface characteristics and material used, the nanoparticles can be developed into smart systems, encasing therapeutic and imaging agents as well as bearing stealth property. Further, these systems can deliver drug to specific tissues and provide controlled release therapy. This targeted and sustained drug delivery decreases the drug related toxicity and increase patient’s compliance with less frequent dosing. Nanotechnology has proven beneficial in the treatment of cancer, AIDS and many other disease, also providing advancement in diagnostic testing.
This paper is excellent and worth a good study.
6. Conclusion: Nanotechnology is truly a multidisciplinary science where chemists, physicist, biologists and pharmaceutical scientist all have played major roles to develop novel treatment and diagnosing modalities. It is evident through this review that application of nontechnology in drug delivery and medicine has paved new pathways and opened many doors for providing customizable and safer treatment option. The treatment of cancer and HIV/AIDS, non-invasive imaging as well as nutraceutical delivery have all progressed with the application of nanotechnology. Ultimately, through the manipulation of molecular size and surface properties, researchers are able to deliver drugs for longer period of time with less frequent dosing (sustained release) and with greater precision and penetration in difficult to access tissues.